FastQCFastQC Report
Sun 14 Apr 2019
SRR6575528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706393
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57670.8164010685270097No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34800.49264361339934No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33030.46758673995920114No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21040.2978511961471872No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14380.20356940116903763No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12770.18077755583648197No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12410.17568124259441983No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12120.17157587914942532No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC11800.16704582293425896No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11680.1653470518535716No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA11350.1606754313816813No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10840.15345565428875993No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10400.1472268269929062No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10190.1442539776017033No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9800.13873297158946932No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA9750.13802515030584958No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9710.13745889327895378No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9670.136892636252058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.1308053732129282No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9150.12953129490241266No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA9130.12924816638896477No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA9020.12769095956500137No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8750.12386872463345475No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8250.11679051179725734No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA7980.11296827686571073No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA7600.10758883511020069No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7570.10716414234002884No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA7470.10574849977278936No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7190.10178470058451881No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT7110.10065218653072723No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT7090.10036905801727933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18750.046.7157671
ATCAACG20100.042.5504233
CAACGCA20900.040.921695
TATCAAC21150.040.6601752
GGTATCA4300.040.521461
TCAACGC22300.039.1955344
AACGCAG23250.037.189766
GTACATG36000.036.4966931
TACATGG36500.035.66282
ACATGGG37050.035.133393
AGGTATA8500.033.1711736
CATGGGA17150.032.8810464
TAGGTAT8600.032.7854655
ACGCAGA26100.032.5886657
TTAGGTA8900.031.6803344
GTGCAAG3500.030.9465051
CATGGGG20550.030.8709814
CGCAGAG27600.030.8393738
GGTATAG9300.030.317747
GCAGAGT28000.030.2308649
Produced by FastQC (version 0.11.2)