FastQCFastQC Report
Sun 14 Apr 2019
SRR6575527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725489
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA56930.7847121045253616No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34370.4737494296950058No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34220.47168185871874No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22500.31013564643984953No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13920.19187058659745357No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13050.17987867493511273No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12280.16926514392361566No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11740.16182188840905926No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11700.16127053614872178No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC11020.15189754772298408No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10790.1487272722260434No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA10410.14348942575283705No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10150.13990563606064324No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10000.13783806508437757No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9580.1320488663508337No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9500.1309461618301587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.13053264763490555No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9420.12984345730948368No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9340.12874075278880864No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA8980.12377858244577106No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8960.1235029063156023No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8950.12336506825051793No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8940.12322723018543355No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA8480.11688667919155218No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA8220.11330288949935836No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7620.10503260559429571No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA7620.10503260559429571No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA7590.10461909139904257No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT7410.10213800622752378No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7260.10007043525125812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC200.002163009570.497119
GTATCAA16100.044.2497251
GGTAATC3500.040.2840588
GTGCAAG4000.037.7441331
GTACATG33900.037.71631
GGTATCA4050.037.278161
TACATGG35450.035.9280052
ATCAACG19100.035.9252243
ACATGGG35550.035.430333
AAGGGTA3850.035.4011425
CAACGCA19550.035.0983055
TATCAAC20200.034.6668972
TCAACGC20800.032.989034
AGGTATA10000.032.898656
TAGGTAT10000.032.898655
CTTAGGT10000.032.898653
AACGCAG22050.032.8240476
CATGGGA16550.032.657274
TTAGGTA10200.032.253584
CATGGGG20850.032.0082784
Produced by FastQC (version 0.11.2)