FastQCFastQC Report
Sun 14 Apr 2019
SRR6575526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380009
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.13736516766708157No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5000.13157583109873713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGA250.005231581656.4048294
GTATCAA8600.046.032051
GTACATG20250.039.564291
TACATGG20300.038.4367752
ACATGGG20850.037.4228633
AACGCAG11400.034.2177166
ATGGGTA2750.034.180245
ATCAACG11450.033.6622623
CATGGGT5350.033.3816384
TCAACGC11600.033.2226034
CAACGCA11700.032.938655
CATGGGA10300.031.9402734
GGGTACC2650.030.1495517
GGTACCT2500.030.0786118
TATCAAC13250.029.4440232
GGTATCA4750.028.7729171
TAATACT1503.6858182E-828.1986984
GCTCTAT856.7001814E-427.7223831
TTATGCA856.809475E-427.645784
CGCAGAG14150.027.5676338
Produced by FastQC (version 0.11.2)