FastQCFastQC Report
Sun 14 Apr 2019
SRR6575517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790269
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30790.38961416935246096No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16940.21435738969895063No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15250.19297226640548976No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11440.14476083460188874No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8370.10591330293861963No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8350.1056602245564485No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8270.10464791102776395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.057.8811
GGTATCA3650.050.3316731
ATCAACG19300.045.5311363
TCAACGC19200.045.523534
CAACGCA19650.044.4810075
AACGCAG20200.043.269896
TAGGTAT4950.039.872025
AGGTATA4850.039.725226
GTCTTAG5700.039.6676061
TATCAAC23300.037.9163132
CTTAGGT5100.037.7779053
TTAGGTA5350.036.8909384
GGTATAG5500.035.8848237
ACGCAGA24400.035.821797
GTACATG34450.034.320541
GTATAGT5900.034.2722788
TACATGG35150.033.9572532
CGCAGAG26100.033.6920668
GTACATA1001.3859808E-632.9736981
ACATGGG36450.032.6172373
Produced by FastQC (version 0.11.2)