FastQCFastQC Report
Sun 14 Apr 2019
SRR6575516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849394
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32500.3826257308151459No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16300.19190152037805777No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16080.18931143850792448No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11430.13456652625283436No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8850.10419192977581664No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8620.10148411691158638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.057.9282231
GTATCAA15400.052.6620181
TCAACGC18750.042.6167224
ATCAACG19000.042.0559773
CAACGCA19350.041.2928435
AACGCAG19950.040.286556
TATCAAC21900.036.272292
TACTAGA801.1576622E-535.2528042
GTACATG35250.034.6441881
AAGGGTA3000.034.467385
TACATGG35050.034.3308332
TAGGTAT5650.033.2750245
ACGCAGA24600.032.6675577
ACATGGG36300.032.6306953
AGGTATA5800.031.60416
TAGAGTA902.592766E-531.3339825
GTCTTAG6650.030.4885371
TTAGGTA6500.030.3716454
CGCAGAG27300.029.6088458
GGTAATC3500.029.539998
Produced by FastQC (version 0.11.2)