FastQCFastQC Report
Sun 14 Apr 2019
SRR6575515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235234
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5630.23933615038642372No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3280.13943562580239252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.13433432241937815No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.12328149842284705No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2860.12158106396184226No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2780.11818019503983268No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2690.11435421750257192No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2680.11392910888732072No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2660.11307889165681831No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2550.10840269688905517No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2510.10670226242805038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480.10542693658229677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG250.005221044656.4268079
GTATCAA8700.042.166931
GTATAGA500.001693540537.6258771
AACGCAG10050.037.398896
CAACGCA10850.034.641375
TCAACGC10750.034.5265664
ATCAACG10800.033.9317053
GTACATG16600.032.0159951
ACGCAGA11650.031.8660567
GTGCTAT753.2545213E-431.3548981
GCGCATT300.00416182231.32822862-63
ATGGGCG600.004161959531.3215685
ACATGGG16700.031.2277953
TACATGG17050.030.8688722
TATCAAC12000.030.5450232
TGGGTAC2000.030.538536
CGCAGAG12200.030.4488938
GGTACCT2101.8189894E-1229.1090688
TATACCT650.00615563828.9122185
GCAGAGT13100.028.3569839
Produced by FastQC (version 0.11.2)