FastQCFastQC Report
Sun 14 Apr 2019
SRR6575510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037710
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43430.41851769762264984No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22880.2204854920931667No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21810.21017432616048798No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14200.13683977219068913No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10690.10301529329003287No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10540.10156980273872276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.054.1713261
TCAACGC19850.047.3472254
ATCAACG20100.046.758333
GGTATCA4650.046.5586171
CAACGCA20750.045.520085
AACGCAG22150.042.6429676
TATCAAC23100.040.69172
GTACATG36650.032.4894031
ACGCAGA28850.032.4139757
TACATGG37950.031.8279882
CGCAGAG30200.030.985918
ACATGGG39050.030.6862773
ACCTAAG15050.029.3958871
CTAAGAC16750.027.7744463
TAAGACA19300.027.5135234
GCAGAGT34250.027.4591799
CCTAAGA17650.026.8945642
ATTATCC13900.026.0316093
GTCTTAG9000.025.624111
CATGGGG26500.025.5353414
Produced by FastQC (version 0.11.2)