FastQCFastQC Report
Sun 14 Apr 2019
SRR6575508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473113
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8010.16930416200780787No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6890.145631170566017No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6790.1435175106158571No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6510.1375992627554094No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT5800.1225922771092741No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5740.12132408113917817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.11878768919898629No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5310.11223534335349061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.052.6944271
CAACGCA14850.043.34935
ATCAACG15300.042.0743183
ACCTAAG2200.040.59781
AACGCAG17650.038.0696876
TATCAAC18050.036.4604872
TCAACGC18250.035.7882044
CCTAAGA2700.034.820732
ACGCAGA18500.034.79667
CGCAGAG18600.034.6095248
GGTATCA4800.033.297321
GCAGAGT20150.031.9472489
CTAAGAC2800.031.884793
TAAGACA3350.030.8578624
GTACTAG2600.028.927991
GTACATG19950.027.5685921
AGAGTAC18750.026.9398510-11
ATAAGGT2104.9112714E-1126.850353
CAGACTA3500.026.8503489
ACATGGG20500.026.8176043
Produced by FastQC (version 0.11.2)