FastQCFastQC Report
Sun 14 Apr 2019
SRR6575507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519342
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8390.1615505774614801No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7480.1440284051742397No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7440.1432581997989764No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6010.11572335763331291No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5850.11264253613225968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.10185966087857326No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5250.10108945550330996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12250.051.9278871
CAACGCA14600.041.5249755
ATCAACG15200.040.504213
TATCAAC17100.037.652772
GGTATCA4900.037.5034751
TCAACGC16850.036.5379264
AACGCAG17050.036.384976
GTACTAG2500.033.926221
ATTAAGT702.1841085E-433.569473
ACGCAGA18600.033.1002167
TATTAAG1001.4098332E-632.898082
CGCAGAG19100.031.9876638
GCATTAA600.004107417531.413171
TACATGG21700.030.5373862
GCAGAGT20150.030.3208129
TAGTACT3900.030.1264464
GTACATG22400.029.66021
ACATGGG22500.029.033863
AGGTATA1503.691639E-828.1983556
GGTACCT2500.028.1983558
Produced by FastQC (version 0.11.2)