FastQCFastQC Report
Sun 14 Apr 2019
SRR6575504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259378
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6420.24751520946263753No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3780.14573325416959032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.13069728350129925No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3260.1256852932785356No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2980.11489023741412148No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2620.10101087987416049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC500.00169874237.603863
GTATCAA8000.036.4850161
ATGGGTA2500.035.7236635
CATATGG550.00271434934.1787349
GGTACCT2300.032.6927038
GTATACA600.00412173931.3849621
TACATGG16600.029.4488052
GCCGTAA404.779834E-429.37234762-63
ACATGGG16200.029.3054753
GTACATG16450.029.1908741
ATGGGAG2101.8189894E-1229.098225
GGGTAGA1154.126685E-628.6558361
ATGGGAT1503.6709025E-828.2028925
CATGGGT3850.028.0808034
AACGCAG11100.027.9488126
ATCAACG10750.027.1097563
CAACGCA10950.027.0438695
TCAACGC10850.026.85994
TATTTAC1057.394649E-526.8598962
TACGAGC1057.404364E-526.854728
Produced by FastQC (version 0.11.2)