FastQCFastQC Report
Sun 14 Apr 2019
SRR6575504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259378
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6950.26794870806313564No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4000.15421508377734425No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3590.1384080376901665No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3270.12607083098797892No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3040.11720346367078165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.11219147344801794No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2770.10679394551581091No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2720.10486625696859408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT2100.042.5223278
GTATCAA9350.039.7098461
TAACCAC608.8069995E-539.16536
TGGGTAC2500.037.598696
AACGCAG10850.035.51956
ATGGGTA2550.035.0183875
CATGGGT3250.034.7064824
TCAACGC11000.033.753374
ATCAACG11100.033.4492843
CAACGCA11500.033.1031955
ACATGGG16250.032.971163
TACATGG16600.032.8422282
GTACATG16950.031.8867931
ACGCAGA12000.031.7238927
CGCAGAG12250.031.076478
GGGTACC3100.030.3215247
CATGGGG6950.029.7543564
ATAACGC804.771772E-429.3739783
ATCTAAT650.00614655228.9220681
CTTACAC856.8031525E-427.6460933
Produced by FastQC (version 0.11.2)