FastQCFastQC Report
Sun 14 Apr 2019
SRR6575496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461989
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41910.9071644562965785No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21740.47057397470502543No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19870.43009682048706793No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17460.377931076281037No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12750.27598059694061977No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12740.2757641415704703No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11590.25087177400327715No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11090.24004900549580185No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11010.2383173625346058No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9100.1969743868360502No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8970.19416046702410664No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7750.16775291186586694No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7330.1586617863195877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.15628077724794315No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA7130.15433267891659758No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG6880.14892129466285994No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6760.14632383022106588No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG6740.14589091948076688No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6540.14156181207777674No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6360.13766561541508562No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA6270.13571751708374008No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA6230.13485169560314206No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6070.13138840968074997No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA6060.13117195431060047No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC5920.12814157912850738No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT5880.12727575764790935No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA5720.12381247172551728No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT5580.1207820965434242No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA5260.11385552469864002No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG5230.1132061585881915No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT5170.11190742636729446No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT5160.11169097099714496No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5120.11082514951654694No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG5120.11082514951654694No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG5060.10952641729564991No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5020.10866059581505189No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT4990.10801122970460336No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA4930.10671249748370633No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA4920.10649604211355683No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT4890.1058466760031083No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC4870.1054137652628093No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC4870.1054137652628093No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA4790.10368212230161325No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA4640.10043529174937064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.069.421381
GGCGAAA301.369712E-462.672081
GTATCAA14850.049.3780021
ATAGCGT450.001013434441.7768676
TATCAAC16900.041.7195782
AAGGGTA3300.041.3021285
ATCAACG17150.041.106973
TCAACGC17300.040.7505534
CAACGCA17350.040.6331185
GTGCAAG4050.039.4601971
AACGCAG17900.039.3846136
GGTAATC3450.038.1440938
CTATTAA1251.5097612E-1037.603251
CTTAGGT6000.036.815863
AGGGTAA3600.036.5547566
TTAGGTA6100.036.2123224
TAGGTAT6300.035.0627255
TCTTAGG6500.033.9875532
GTCTTAG6850.032.9371531
ACGCAGA21600.032.6381767
Produced by FastQC (version 0.11.2)