FastQCFastQC Report
Sun 14 Apr 2019
SRR6575430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832867
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38030.45661552204613703No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23890.28684051595272714No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22070.2649882874456546No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13540.16257097471745188No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13320.15992949654626729No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10830.13003276633604166No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10260.12318893652888156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.054.468131
GGTATCA3950.052.3997151
TCAACGC19500.044.8237534
ATCAACG20100.043.7195243
CAACGCA20400.042.8462375
AACGCAG20950.041.7213976
TATCAAC21900.040.986912
GGTAATC3250.037.621228
CTTAGGT7750.035.168693
TAGGTAT7700.034.7867625
TTAGGTA8050.033.8580554
AGGGTAA3650.033.4742166
GTCTTAG8750.033.3316651
ACGCAGA26100.033.3089647
AGGTATA8100.033.0688976
GTACATG32700.031.3604371
TATTCGC300.004164700431.32843424-25
TACATGG33000.030.9030782
AAGGGTA4000.030.545225
CGCAGAG28350.030.5216248
Produced by FastQC (version 0.11.2)