FastQCFastQC Report
Sun 14 Apr 2019
SRR6575429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937430
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39490.4212581206063386No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24960.26625988073776174No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23370.24929861429653413No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14170.15115795312716682No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14030.14966450828328515No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11980.12779620878358916No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11110.11851551582518162No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9590.10230097180589483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.056.9467241
GTATCAA20600.052.6369671
ATCAACG22300.047.4175453
TCAACGC22300.047.4175454
CAACGCA23250.045.480055
AACGCAG24150.044.1743436
TATCAAC24350.043.232512
ACGCAGA31100.034.3025867
GTACATG39000.033.4846271
CGCAGAG32200.033.2767078
TACATGG39300.033.004862
TTAGGTA8700.031.8708844
TAGGTAT8850.031.3307025
ACATGGG41100.031.33073
GTCTTAG9500.031.2641941
AGGTATA9000.030.8085236
GCAGAGT34750.030.4290989
CTTAGGT9150.030.3034673
GGTATAG9450.029.8387627
GTATAGT10200.029.0269728
Produced by FastQC (version 0.11.2)