FastQCFastQC Report
Sun 14 Apr 2019
SRR6575415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96419
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2490.25824785571308556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.13897675769298581No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1340.13897675769298581No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1100.11408539810618239No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1080.11201111814061544No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1040.10786255820948154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC250.005234103656.365773
GGGTACC1001.2732926E-1146.9714747
GGTACCT1052.1827873E-1144.7347378
TGGGTAC1052.1827873E-1144.7347376
CTATTCC608.7954526E-539.1428954
ATGGGTA1251.4915713E-1037.577185
TAGGAGC500.001699364937.577186
CATGGGT1750.034.8930934
ACACAGC550.002712834.161076
GATTGCC300.00414372131.34683450-51
CCATGCG600.00415459931.3143169
GTACCTG1551.4915713E-930.3041769
ACATGGG6700.030.145873
GTACATG7100.029.78611
TACATGG7150.029.5778082
GTACAGA650.00612925128.9205231
TCTACAC650.006144821628.9055213
GTATCAA4000.028.197511
CATGGGG2700.027.8349464
AACGCAG4900.024.923645
Produced by FastQC (version 0.11.2)