FastQCFastQC Report
Sun 14 Apr 2019
SRR6575404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417547
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8310.19901951157594236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.15231818214476453No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5730.13723006032853785No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5190.12429738448605784No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5040.12070497452981342No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4630.11088572064941193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.053.0691831
TCAACGC15900.039.3137664
ATCAACG15850.039.141263
CAACGCA16100.038.8253975
AACGCAG16500.037.8841746
TATATCT702.1828574E-433.570834
ACGCAGA19300.031.9009867
GTACATG24600.031.550281
ACATGGG25000.031.0194453
TATCAAC19900.030.9651072
TACATGG25100.030.9217852
CGCAGAG20000.030.7844528
GGTATCA5350.030.7729131
GTATAAG1701.200533E-1030.4367391
GTATACA650.006150278328.922564
GCAGAGT21450.028.7034539
CATGGGG11600.028.3615634
CCGACCA1503.686546E-828.1994979
CATATAG856.8094785E-427.6465663
TTATGGG856.8094785E-427.6465664
Produced by FastQC (version 0.11.2)