FastQCFastQC Report
Sun 14 Apr 2019
SRR6575393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451814
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9260.2049515951254277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.15448835140124034No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5830.12903539952281248No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5750.12726475939213924No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4940.10933702806907267No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4640.102697127579048No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4630.10247579756271386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.056.8645481
TCAACGC16400.042.1072654
CAACGCA16900.040.861495
ATCAACG16950.040.740963
AACGCAG17400.039.957296
GTACGTA651.4107133E-436.1600231
ACGCAGA20300.034.2491077
TATCAAC20550.034.083682
GTATTAC702.1807215E-433.5771641
CGCAGAG20900.033.4906468
GGTATCA4900.032.6178171
GCAGAGT22650.030.6956679
ATGGGTA4050.030.1579555
CATGGGT6350.028.85194
GTACATG25300.028.6135851
GTCTAGG1005.3102915E-528.2048171
ACATGGG25600.028.0759873
TACATGG25850.028.0047842
AGAGTAC21900.026.27698510-11
TAGACGA950.001307113924.724649
Produced by FastQC (version 0.11.2)