FastQCFastQC Report
Sun 14 Apr 2019
SRR6575389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615085
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51750.8413471308843493No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31470.5116366030711202No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31080.505296015997789No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20690.3363762731980133No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20280.32971052781322907No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15610.2537860620889796No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15530.252485428843168No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12980.21102774413292472No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12630.2053374736824992No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12180.1980214116748092No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11920.19379435362592162No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11880.19314403700301583No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11700.19021761219993985No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11450.18615313330677874No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11280.1833892876594292No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11130.18095060032353252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.17022037604558718No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9790.15916499345618898No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT9770.1588398351447361No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA9240.15022313989123454No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8720.14176902379345943No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT8670.1409561280148272No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8630.14030581139192144No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8520.13851744067893054No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8310.13510327840867523No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG7970.12957558711397613No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7460.12128405017192746No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG7320.11900794199175724No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7050.11461830478714323No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA6940.11282993407415234No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA6930.11266735491842592No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA6930.11266735491842592No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT6930.11266735491842592No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG6840.11120414251688791No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC6810.11071640504970857No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC6710.10909061349244414No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT6680.1086028760252648No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG6650.10811513855808547No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC6500.10567645122218881No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC6430.10453839713210368No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA6250.10161197232902769No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA6210.10096165570612192No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA6200.10079907655039548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.069.143131
GTATCAA21050.049.3626061
TATCAAC21850.047.1249122
ATCAACG21900.046.998213
GGTAATC4750.046.5034948
TCAACGC22200.045.9396934
CAACGCA22800.044.936895
AACGCAG23100.044.1498376
GTGCAAG5800.040.5321771
TAGGTAT9500.039.0827265
TGCAAGG6150.038.9899752
TTAGGTA9650.038.475224
CTTAGGT9900.038.453083
AAGGGTA6000.038.381875
AGGGTAA5950.037.9145246
GTCTTAG10550.037.8812561
GTAATCA6000.037.5985689
AGGTATA9900.037.5036246
GGGTAAT5950.037.1246387
ACGCAGA27600.036.9514927
Produced by FastQC (version 0.11.2)