FastQCFastQC Report
Sun 14 Apr 2019
SRR6575388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529052
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47770.9029358172731603No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31330.592191315787484No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23580.44570287986814155No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16310.3082872761089647No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15810.29883640927545874No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14010.2648132886748373No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13730.2595208032480739No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12050.22776589068749387No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA11630.21982716254734885No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10850.20508381028707953No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10700.20224855023702773No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10660.20149248089034727No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT10530.19903525551363568No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10390.19638901280025403No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9610.18164566053998474No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9130.172572828379819No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8760.16557918692302456No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8530.16123178817961184No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7720.14592138390933218No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG7700.14554334923599194No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7610.14384219320596087No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA7430.1404398811458987No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7420.14025086380922858No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7290.13779363843251705No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT7200.13609248240248595No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG6960.1315560663224031No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6920.13079999697572262No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG6870.129854910292372No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA6300.1190809221021752No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT6230.11775780074548436No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA6130.11586762737878317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.11567861004211305No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG6040.11416647134875212No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA5950.11246531531872103No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC5890.11133121129870031No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG5860.11076415928868996No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC5800.10963005526866923No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT5780.109252020595329No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC5770.10906300325865888No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC5760.10887398592198877No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA5750.10868496858531865No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT5610.10603872587193698No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA5410.10225837913853458No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA5370.1015023097918541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.066.177991
TCAACGC14400.046.340514
ATCAACG14350.046.1744963
GTATCAA14500.046.0862581
TATCAAC14900.045.100852
CAACGCA15000.044.4868935
GTGCAAG4150.043.0911
AACGCAG15350.042.8602456
GTCTTAG10250.040.8617741
TAGGTAT10100.040.4792865
CTTAGGT10050.040.213083
TTAGGTA10150.039.816894
AGGTATA10300.039.2370386
GGTAATC3300.038.474438
AAGGGTA3600.037.855635
TCTTAGG11150.037.510262
GGTATAG11050.036.5738877
ACGCAGA18550.035.4665647
CGCAGAG19150.034.3780828
AGGGTAA4200.033.566576
Produced by FastQC (version 0.11.2)