FastQCFastQC Report
Sun 14 Apr 2019
SRR6575359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298245
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.20050629516001947No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3590.12037083605760364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.053.036441
ATCAACG10500.045.206063
TCAACGC10500.045.206064
CAACGCA10800.044.3854875
AACGCAG11400.042.0494046
TATCAAC11850.041.245782
GGTATCA3850.039.1604271
ACGCAGA13350.035.9073567
CGCAGAG13800.034.7364658
GTACATG14250.032.7324521
TACATGG14100.032.3308532
GCAGAGT15700.030.5326889
ACATGGG14350.030.4575963
ACCGTCG650.006149264528.9208588
AGCCCGT700.00882902526.8550836
GTATAAT1701.2085184E-724.9431781
AGTACTT7300.024.14198312-13
CATGGGG6300.023.8711854
AGAGTAC14350.023.25257310-11
ATGGGTA2653.6379788E-1123.0548345
Produced by FastQC (version 0.11.2)