FastQCFastQC Report
Sun 14 Apr 2019
SRR6575359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298245
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.15591208570135293No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3720.12472966856108232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC301.4539983E-678.328733
TCTAACG200.002143275770.649852
CCGCTAA200.002161909870.495854
CCCGCTA200.002161909870.495853
CGCTAAA250.00523346756.3966835
CTAGGAC901.3089448E-841.7753143
GTATCAA7700.041.594721
TGCCCGC1204.098183E-935.3249241
GTACATG15750.034.6894531
TACATGG16100.033.0576932
AACGCAG10200.032.2530026
CAACGCA10000.031.958125
ATCAACG10050.031.7991243
TCAACGC10100.031.6417034
ACATGGG16700.031.5191023
ATACGAC300.004160589531.3314932-33
GGTATCA3700.030.5512871
TATCAAC11150.028.7246042
CATGGGG7400.027.9443024
ACGCAGA12300.026.7463937
Produced by FastQC (version 0.11.2)