FastQCFastQC Report
Sun 14 Apr 2019
SRR6575348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328579
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.19082168976106204No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3490.10621494374260072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG250.0052360156.391059
GTATCAA8600.047.164811
TCAACGC10700.038.208894
ATCAACG10700.037.7697073
CAACGCA11000.037.5940325
AACGCAG11300.037.0118266
TATCAAC12000.034.8634152
GGTATCA4150.034.0950431
GTACATG15850.032.7326741
TACATGG15800.032.7262882
ACATGGG16250.032.099523
CGCAGAG13150.031.804848
ACGCAGA13300.031.4461367
GTATACG600.004086070731.4432051
GGTACCT2400.031.3283638
CCTATAC600.004159926431.3283633
ATGGGTA2600.030.7258935
GGGTACC2350.029.9952417
CATGGGT4500.029.2398034
CATGGGG6000.027.4123154
Produced by FastQC (version 0.11.2)