FastQCFastQC Report
Sun 14 Apr 2019
SRR6575348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328579
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.14182281886547832No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4120.12538841496261172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA352.9323527E-453.721943
GTATCAA9150.048.81221
CCGCTAA405.6710374E-446.9995464
CGACGGT757.435656E-637.5939147
AACGCAG12000.037.5939146
CAACGCA12050.037.0479435
TCAACGC12100.036.900474
ATCAACG12150.036.7542083
TATCAAC12550.035.962792
CTACGAC801.1562121E-535.2496574
CGCTAAA550.002716354334.176285
TACGACG851.7529279E-533.17115
ACGACGG851.7529279E-533.17116
ACGCAGA14100.031.661547
GTACATG17450.031.2527621
CGCAGAG14600.030.5772428
TACATGG17650.030.099522
ATGGGTA2500.030.075135
AAACGGT650.00615272628.91839494
TACTAGC650.00615272628.9183947
Produced by FastQC (version 0.11.2)