FastQCFastQC Report
Sun 14 Apr 2019
SRR6575345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641595
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22670.35333816504180987No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14430.22490823650433683No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13890.21649171206134712No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7930.12359822006094187No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6850.10676517117496241No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6850.10676517117496241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11600.052.764171
GGTATCA3600.052.3131941
TCAACGC13100.045.9176064
ATCAACG13450.045.0721243
CAACGCA13550.044.392675
AACGCAG14350.042.24536
TATCAAC14850.041.145772
GTACATG23350.033.8747561
GTCTTAG5900.033.515911
TACATGG24050.032.832392
ACGCAGA18400.032.435947
ACATGGG24200.032.2354163
CGCAGAG19000.031.4336978
ACCTAAG8200.031.0051381
CTAAGAC9300.029.8132723
GGTAATC1951.8189894E-1128.9395668
ACCTCGC650.00613506328.9395648
ACCAGAT10450.028.79885394
AGGTATA4800.028.3920926
GCAGAGT21050.028.3724619
Produced by FastQC (version 0.11.2)