FastQCFastQC Report
Sun 14 Apr 2019
SRR6575344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712033
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22120.3106597587471367No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15320.21515856708888495No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14970.21024306457706315No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9240.12976926631209507No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8300.11656763099463087No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7530.10575352546862295No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7370.10350643860607585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.10154023760134713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14650.053.9084431
GGTATCA4300.053.5689051
TACTAAG503.0046918E-547.009452
TCAACGC16650.046.8584064
ATCAACG16950.046.029053
CAACGCA17250.045.2285465
AACGCAG17850.043.9715656
TATCAAC19550.040.1564062
ACGCAGA22750.034.5007637
CGCAGAG22900.034.2747768
TACATGG26800.030.5210592
GCAGAGT25850.030.3633429
GTACATG27200.029.899391
ACATGGG28000.029.0390033
GGTAATC2800.028.5354358
CTAAGCG856.8055216E-427.6526152
AAGGGTA3400.026.2644525
TAAGCGA909.5146487E-426.1108573
AGAGTAC22250.025.98177110-11
AGGTATA4300.025.139296
Produced by FastQC (version 0.11.2)