FastQCFastQC Report
Sun 14 Apr 2019
SRR6575336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705395
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24000.34023490384819854No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17050.24170854627549107No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14860.21066211129934292No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA8920.1264539725969138No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7920.11227751826990552No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7780.11029281466412436No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7580.10745752379872271No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC7290.1033463520438903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.058.3424531
GTACGGT250.005237407556.3940056
GTATCAA13800.050.482751
TCAACGC13600.049.064834
ATCAACG13700.048.7066963
CAACGCA14000.048.334295
AACGCAG14350.046.8312576
TATCAAC15750.042.668482
ACGCAGA19400.034.6406487
CGCAGAG20300.033.591678
AGGTATA5150.031.0258276
GTACATG24050.030.9244771
GCAGAGT22650.030.7293369
TAGGTAT5200.030.7253255
TACATGG24750.030.1907272
GTCTTAG6150.029.850381
TTAGGTA5550.029.6343864
TAAGGCG804.7621917E-429.3927129
GTGCAAG4350.029.216951
ACATGGG25200.028.9036053
Produced by FastQC (version 0.11.2)