FastQCFastQC Report
Sun 14 Apr 2019
SRR6575335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792618
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24670.3112470319876662No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16800.21195582235074145No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16780.2117034939908001No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10000.12616417997067944No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8650.10913201567463772No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8600.10850119477478432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.058.342051
GTATCAA14500.051.6721731
TCAACGC15950.045.9691774
ATCAACG16500.044.1520163
CAACGCA16650.044.0365375
AACGCAG17250.042.77466
TATCAAC17200.042.628392
CTTAGGT6150.032.8621643
ACGCAGA22400.032.3108337
GGTAATC2350.031.9983488
TCTATAC1351.3258614E-831.3336893
AGGTATA6300.031.3317136
TAGGTAT6550.030.8553125
CGCAGAG23500.030.7984098
TTAGGTA6750.030.6373844
GTCTTAG7350.030.1327061
GGTATAG6800.029.027917
GTACATG27100.028.3430061
ACCTAAG9050.028.1172471
ACCAGAT13750.028.02941194
Produced by FastQC (version 0.11.2)