FastQCFastQC Report
Sun 14 Apr 2019
SRR6575328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585369
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22110.3777104698062248No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13670.2335279114541426No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12820.21900715616986893No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8270.14127840729522745No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7150.12214517680300802No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5900.10079112491437024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.058.1609761
GGTATCA3450.050.4838071
ATCAACG12850.049.730813
TCAACGC12850.049.730814
CAACGCA13450.047.162975
TATCAAC14750.044.9176252
AACGCAG14250.044.844976
ACGCAGA16900.037.81637
ACCTAAG7350.036.505391
CGCAGAG17950.035.6315968
CTAAGAC8450.034.476623
GTACATG21250.031.89871
GTACTAT600.00412796331.3818241
AATCGAC300.004166086731.32553516-17
CCTAAGA9000.031.3255312
CTTAGGT4100.030.9435123
TACATGG21700.030.5315672
TAAGACA11600.030.3803654
GCAGAGT20900.030.3772419
ACCAGAT10450.030.14706894
Produced by FastQC (version 0.11.2)