FastQCFastQC Report
Sun 14 Apr 2019
SRR6575328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585369
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22480.38403126916526154No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14610.2495861584743982No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12910.22054464790585085No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8490.1450367204276277No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7190.12282850646344443No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6180.10557443253742511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.054.54781
GGTATCA2250.050.1425861
ATCAACG12750.043.4874843
TCAACGC12750.043.4874844
CAACGCA13550.040.916465
AACGCAG14150.039.1814886
TATCAAC15450.036.207332
AGGTATA4300.036.0579346
TAGGTAT4350.035.643485
GTCTTAG5200.035.2565041
ACGCAGA16450.033.7032287
TTAGGTA4750.033.633944
ACCTAAG7900.032.7275621
CTTAGGT4900.032.6043323
ACCAGAT9150.031.83926894
CGCAGAG17550.031.5907758
CTTAAGT1807.2759576E-1231.3257313
GTACATG20500.030.7276231
GGTATAG5050.030.7027977
CTAAGAC8900.030.6217823
Produced by FastQC (version 0.11.2)