FastQCFastQC Report
Sun 14 Apr 2019
SRR6575264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719700
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37560.5218841183826595No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24020.3337501736834792No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17910.24885368903709879No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12690.1763234681117132No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12580.1747950534945116No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11350.15770459913852994No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10470.14547728220091705No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8440.1172710851743782No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7930.11018479922189801No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7760.10782270390440461No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7630.10601639572043907No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7620.10587744893705711No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7560.10504376823676533No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7350.10212588578574407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.066.2252041
GTATCAA15800.051.8624341
TCAACGC16350.047.9979934
CAACGCA16600.046.708965
ATCAACG17050.046.0273973
AACGCAG17400.044.5645186
TATCAAC18600.042.700042
GTAATAC450.001014547741.7707063
GTACTAA500.001685077237.674781
ACGCAGA22000.035.2440347
CGCAGAG22250.034.6609158
AAGGGTA3300.034.1760335
CTATATT550.002718592534.176034
GTACATG30050.033.3807331
GTAATCA4100.033.2613839
TACATGG30200.032.9900862
GGTACCT3000.032.91738
GCAGAGT23550.032.7475749
ATAGTAC1457.4396667E-1032.4083063
GTGCAAG4850.032.042981
Produced by FastQC (version 0.11.2)