FastQCFastQC Report
Sun 14 Apr 2019
SRR6575263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795891
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37190.4672750414315528No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23570.29614608030496636No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18980.2384748665332313No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13440.16886734490024388No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12960.16283636829666373No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11280.14172795018413326No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11010.13833552584461944No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9460.11886049722889189No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8640.1085575788644425No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC8300.10428563710357323No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8080.10152143949359899No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7980.1002649860345198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.058.6342051
GTATCAA16350.052.7362061
ATCAACG18300.046.4876483
TCAACGC18800.045.7512864
CAACGCA19500.043.86795
AACGCAG20550.041.3977556
TATCAAC22400.038.8180582
CGGCGAT757.4424897E-637.59869494
ACGCAGA24800.034.3012287
ACCTAAG13150.034.0387341
CGCAGAG25050.033.9588978
CTAAGAC14650.032.403643
CCTAAGA14800.031.4400712
AAGGGTA2850.031.3342155
GTACCGT902.5924319E-531.3342136
GTACATG34400.031.091591
GTCTTAG7650.030.7952771
TAAGACA17950.030.6359584
TACATGG35200.029.9099312
TACCGTG1102.9677376E-629.908057
Produced by FastQC (version 0.11.2)