FastQCFastQC Report
Sun 14 Apr 2019
SRR6575259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485012
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.14370778454966063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.12535772310788187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.048.7846681
ATCAACG13700.037.050083
TCAACGC14200.035.7455024
CAACGCA14300.035.166875
AACGCAG14600.034.444266
TACATGG22800.033.194532
GTACATG23100.032.7870981
TATCAAC15950.032.419592
CATGGGG10750.031.4782074
GTCTTAC1052.0476655E-631.3615721
ACATGGG24250.031.2032663
ACGCAGA17050.029.4947937
CGCAGAG17200.029.2375728
GTACTTG2800.028.561431
TTATACC1205.8353E-627.4159154
ACCGTGC700.00883226126.8564058
CAGTACG950.001303706824.7361649
GCAGAGT20850.024.3446589
ATGGGGG4850.023.2570935
GGGTACC3500.022.8279467
Produced by FastQC (version 0.11.2)