FastQCFastQC Report
Sun 14 Apr 2019
SRR6575243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258757
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10680.41274245720888714No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4940.19091270960785603No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4340.1677249311129747No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3380.13062448552116465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.11748474437406524No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3040.11748474437406524No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT2880.11130133677543022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2250.060.713261
GTATCAA5600.054.6755831
ATCAACG7800.038.560693
TCAACGC7900.038.0725824
CAACGCA8250.036.4573825
GTATTAT801.1376276E-535.328841
AACGCAG8650.035.307976
ATTATCC3000.034.4636153
GGTAATC550.00268356834.2582661
GTACATA550.00268356834.2582661
ATATAGA550.00271688634.172196
TATCAAC9100.033.568462
TTAGGTA1850.033.0241054
TATTATC3000.032.8970872
AATATAG600.004156876431.330565
ACCAGAT4450.030.64427694
TAGGTAT2000.030.54735
ACGCAGA9950.030.2284837
TTATCCT3450.029.9683654
CAATACT953.743424E-529.6815854
Produced by FastQC (version 0.11.2)