FastQCFastQC Report
Sun 14 Apr 2019
SRR6575243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258757
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10390.4015350309363611No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5150.19902843208106447No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4310.16656554218823064No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3360.12985155957133526No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3210.12405461494761495No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2940.11362011462491836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1650.068.368691
TGTACTA250.00523794356.3823785
TTAAGTA352.9377805E-453.6975024
GTATCAA6150.050.442761
TCAACGC7050.043.3197984
ATCAACG7100.043.0147253
CAACGCA7150.042.713925
AACGCAG7450.040.99396
TATTAAG704.618956E-640.2886962
ACCTAAG3250.036.1565251
TATCAAC8750.035.4540522
GTGCAAG1353.4197E-1034.817391
TAGGTAT2200.034.171145
CTAAGAC3600.033.9338343
ACGCAGA9050.033.7463577
ATTAAGT702.1843502E-433.560943
CTTAGGT2400.033.281263
GTATTAA851.7486673E-533.178931
CCTAAGA3700.033.0294722
TAAGACA3750.032.5764854
Produced by FastQC (version 0.11.2)