FastQCFastQC Report
Sun 14 Apr 2019
SRR6575242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261217
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9670.3701903015500523No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4510.17265338779635322No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3970.15198092007794287No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3140.1202065715477936No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3030.11599551330885814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.10795622030725413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5050.060.6646841
GGTATCA1300.058.0083541
TCAACGC6400.048.483664
CAACGCA6500.047.7286155
ATCAACG6550.047.3733523
AACGCAG6750.044.568146
GTCTTAT459.986555E-441.8949241
CTGGTCG450.001012891441.7746289
TATCAAC7400.041.2964822
GGTAATC853.9423503E-738.702978
ACGCAGA7900.038.6679737
CGCAGAG8350.036.584078
AAGGGTA906.1602987E-736.55985
CCTTATT651.4079512E-436.1649672
CTTAGGT1451.8189894E-1135.666143
TAGGTAT1353.4197E-1034.818865
AGGTATA1457.366907E-1032.4175576
GTACATG9500.032.2480661
GCAGAGT9800.031.6506779
ACTTAAG600.004098573731.4211961
Produced by FastQC (version 0.11.2)