FastQCFastQC Report
Sun 14 Apr 2019
SRR6575238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712221
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19740.2771611620550363No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13410.1882842544659593No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13150.18463370217952013No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12380.17382245117737333No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11430.16048389474615324No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9940.13956342202771332No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9770.13717652245581077No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9450.13268353502634717No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9410.1321219115976642No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8980.12608445973932247No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8890.12482080702478585No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8170.11471158530849274No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7880.11063981545054134No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7700.10811251002146806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7600.10670845144976068No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7560.10614682802107772No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7300.10249627573463854No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7260.1019346523059556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.045.73821
GGTATCA4100.043.5690421
TAGGTAT4000.035.2491075
CTTAGGT4050.034.8163763
ATCAACG14450.034.804333
CAACGCA14550.034.239685
GTACTAG1651.8189894E-1234.1881521
AAGGGTA2550.033.1756255
TACATGG26750.033.0378952
TTAGGTA4150.032.842544
GTACATG26950.032.6182861
AGGTATA4350.031.3325396
TTAATAC1501.0677468E-931.3325394
ACATGGG28450.031.0593873
ACCTAAG6850.030.8816321
AACGCAG17050.030.8731166
TCAACGC16300.030.563644
CTAAGAC7600.029.6855413
CCTAAGA7300.029.6219252
TATTATC8200.029.237122
Produced by FastQC (version 0.11.2)