FastQCFastQC Report
Sun 14 Apr 2019
SRR6575230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720387
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49190.6828274247036662No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34200.474744824656747No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31430.4362932701450748No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20190.28026602367893927No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12640.1754612451362948No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11760.16324558882933757No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11300.15686013212342811No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9950.13812020483434598No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9860.13687087634840717No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT9760.13548273358625296No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9570.1328452623381599No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9220.1279867626706201No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9200.12770913411818927No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8950.12423877721280369No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG8830.12257300589821861No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8740.12132367741227978No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8730.12118486313606436No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8700.1207684203074181No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8690.12062960603120268No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA8260.11466059215393949No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8180.1135500779442161No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8130.11285600656313899No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8060.11188430662963103No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7360.10216730729455141No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG7250.10064035025618173No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7230.1003627217037509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.054.392111
TCAACGC15350.052.96474
ATCAACG15800.051.456213
GGTATCA2500.050.8672561
CAACGCA16350.050.0126955
AACGCAG16950.048.5162246
TATCAAC19800.041.3012282
ACGCAGA21400.038.427577
GGTAATC3550.037.0894058
CGCAGAG22300.036.9022948
AAGGGTA3650.036.050725
GTAATCA4000.035.268059
AGGGTAA3750.035.0869336
TTAGGTA8600.034.9727944
GTACATG29350.034.3415761
GCAGAGT24150.034.2701269
GTCTTAG9050.033.8282361
TACCGTG702.1865372E-433.5653047
CTTAGGT8850.033.4538463
GTGCAAG4800.033.362011
Produced by FastQC (version 0.11.2)