FastQCFastQC Report
Sun 14 Apr 2019
SRR6575226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.17851407883815695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.16996375496857236No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5350.11157129927384811No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5080.10594059818899969No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5030.10489787576587963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC652.739529E-643.480611
GTATCAA17100.040.2174451
CCTTATA1457.421477E-1032.4109882
GTACATG31700.032.244691
CGCCGGT1755.456968E-1232.225787
TACATGG31700.031.8742142
ATAGACG600.00416034731.3306248
ACATGGG31850.031.1338833
ATCAACG21300.031.1099833
CAACGCA21250.030.9620275
TCAACGC21550.030.7490794
AACGCAG21650.030.389986
CATGGGG13350.030.2745324
ATAGGGG804.7791965E-429.3724583
GGCGTGT804.7791965E-429.3724588
CATGGGT6050.028.7413124
ATGGGTA3100.027.2879645
TATAGGG1057.4170755E-526.8548182
TATCAAC25150.026.5344812
ACGCAGA25050.026.45287
Produced by FastQC (version 0.11.2)