FastQCFastQC Report
Sun 14 Apr 2019
SRR6575226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7580.1580767193450035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.12429251283591304No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5120.10677477612749575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG205.67488E-447.00229388-89
GTATCAA14650.041.1097221
CCCGTAA250.001702350337.5979182-83
CCTATAC757.4373693E-637.597913
CATATAG902.5917796E-531.3315943
ATCAACG19350.031.3315933
TCAACGC19400.031.2508474
CAACGCA19700.030.7749425
AACGCAG19550.030.7706726
ATGGGAG5700.028.0335355
GTACATG29800.027.4729421
ACATGGG30250.027.1885743
TTTGCGT350.00883769326.85565656-57
TATCAAC22700.026.7384382
GACACGT909.512643E-426.1096616
TACATGG31250.026.0476322
ACGCAGA23050.025.6905487
CTAGTAC950.001303898824.735473
CATGGGA14900.023.9718274
CGCAGAG25300.023.5915768
Produced by FastQC (version 0.11.2)