FastQCFastQC Report
Sun 14 Apr 2019
SRR6575215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525467
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8440.16061903031018124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.12503163852344676No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6080.11570660003387465No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5530.10523972009659978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG555.275852E-542.7318151
GTATCAA15550.038.99451
CATATAG550.002720597234.169193
ATGGGTA3900.033.731125
GTACATG32600.033.5955961
ACATGGG32700.032.4711763
TACATGG34400.031.7010462
ATCAACG19350.031.3217543
CTTAGTA600.004166473631.3217549
TCAACGC20100.030.1530344
CAACGCA20400.029.7096085
GGTACCT3500.029.531948
AACGCAG20750.028.982066
CCCGCTA650.00616228528.912393
CATGGGT7050.028.6560754
ATGGGCG1154.2027677E-628.5981245
GGGTACC4000.028.189587
CATGGGG14350.028.156844
CTAGACA856.8258605E-427.6368434
TATCAAC22700.026.919162
Produced by FastQC (version 0.11.2)