FastQCFastQC Report
Sun 14 Apr 2019
SRR6575213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712248
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14430.20259797149307546No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13350.18743471375138998No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12700.17830867899944963No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12370.17367546135615683No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10960.15387898597117858No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10930.15345778436724286No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10520.1477013624467882No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10000.1404005346452359No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9940.1395581314373645No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9610.13492491379407173No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9280.13029169615077896No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9170.12874729026968135No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8890.12481607529961473No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT8240.1156900405476744No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8060.11316283092406017No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7840.11007401916186497No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7770.10909121541934831No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT7770.10909121541934831No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7710.10824881221147689No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7630.10712560793431501No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC7350.10319439296424841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12500.050.8899541
GGTATCA4250.045.4572561
ATCAACG14900.041.6386763
CAACGCA15600.040.0715645
TCAACGC17100.036.281654
AACGCAG18200.036.1547936
GTACTAG2000.032.984231
GGTAATC1001.4106517E-632.8985528
TACATGG26900.030.7517342
GTACATG27600.030.2184721
ACGCAGA20750.030.1239747
ACATGGG27400.029.6759593
TATCAAC22100.029.5618572
CTATTGA2400.029.3737059
CGCAGAG20850.029.3032678
TAGTAGT1452.6564521E-829.1731764
ACCTAAG4500.028.2721981
AGTAGTG1853.274181E-1027.9466765
TCTAATA1354.6294736E-727.8525772
GGCTATT2650.026.6026027
Produced by FastQC (version 0.11.2)