FastQCFastQC Report
Sun 14 Apr 2019
SRR6575212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701992
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33460.47664360847417064No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23040.32820886847713365No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18460.26296595972603676No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12600.17948922494843247No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11400.16239501304858175No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10750.15313564826949594No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8870.12635471629306316No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7700.10968785969070872No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7640.1088331490957162No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7530.1072661796715632No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7120.10142565727244755No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7060.100570946677455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3650.063.1123471
GTATCAA15500.051.865141
ATCAACG15850.047.7265663
TCAACGC15900.047.576484
CAACGCA16650.045.9977875
AACGCAG17200.044.5269286
TATCAAC18700.040.7271962
TAGGTAT7000.036.2459345
ACGCAGA21450.035.7045757
CTTAGGT7200.035.23913
CGCAGAG22300.034.343648
GTCTTAG8250.034.190731
CGTAGAC550.002720411934.171253
TTAGGTA7550.033.6055034
AGGTATA7600.033.384416
GTACATG28950.032.3158531
TACATGG29400.031.6613182
GGTAATC3150.031.3236458
TATAGTA8600.031.1415339
GGTATAG8200.030.9416527
Produced by FastQC (version 0.11.2)