FastQCFastQC Report
Sun 14 Apr 2019
SRR6575204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399303
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.21086743650811537No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5380.13473477534604048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9000.051.257471
TCAACGC11950.038.1478354
ATCAACG12150.037.5198823
CAACGCA12200.037.3661165
AACGCAG12600.036.179896
GGTATCA4500.034.5203361
GTATTAC550.00269425834.2350431
TATCAAC13800.034.0554732
ACGCAGA14650.031.1171767
GTACATG20050.030.9908261
CGCAGAG14900.030.5950748
ACATGGG20200.030.0126513
TACATGG20650.029.8137952
CATGGGG10500.029.5406934
GCAGAGT18200.025.564069
CATGGGT5450.025.007344
ATGGGGA6300.024.6172455
GGTACCT3150.023.8712678
GTCTAAC1000.001740841423.5365921
GATATAG1000.001740841423.5365921
Produced by FastQC (version 0.11.2)