FastQCFastQC Report
Sun 14 Apr 2019
SRR6575193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431296
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.18687861700549044No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5530.12821820744917642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10109066627096007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.056.2141651
ATCAACG13450.041.228253
TCAACGC13550.040.5771684
CAACGCA13850.039.698245
TATCAAC14500.038.5668452
AACGCAG14450.038.3750846
GGTATCA4400.034.3001671
ACGCAGA16750.033.105677
CGCAGAG17250.032.1460848
GTACATG18700.028.7516121
TACATGG19050.027.875242
GCAGAGT20050.027.6568579
ACATGGG19150.026.5027033
CATGGGG10300.024.6372224
GGTCATA1050.00228675222.4584431
ATGGGGA7000.022.1539445
TTACCCC1303.158302E-421.6891774
AGTACTT11000.021.36798712-13
AGAGTAC19200.021.29380210-11
GTCTATA1353.977436E-420.9612141
Produced by FastQC (version 0.11.2)