FastQCFastQC Report
Sun 14 Apr 2019
SRR6575179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772735
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40090.518806576640116No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26210.33918484344568317No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24300.3144674435608585No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17030.2203860314338033No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13580.17573941907639748No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12830.16603363378130925No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11950.15464551236840574No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9660.12501051460073634No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9070.11737529683526694No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8990.11634001307045753No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8620.111551825658214No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8510.11012831048160107No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG7850.10158721942192343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.051.5868721
GGTATCA2900.048.6291281
TCAACGC17200.044.255074
ATCAACG17400.043.746393
CAACGCA18000.042.0271385
AACGCAG18650.040.5623866
TATCAAC20150.038.026452
GGTAATC3000.037.5894938
TACATGG33600.035.6758352
GTACATG33850.035.551221
GTATTAC550.00271414934.1877481
ACATGGG36000.033.0213243
AAGGGTA3650.032.1827855
ACGCAGA24150.031.3245777
GTCATAC1401.8861101E-830.2195281
CGCAGAG25350.030.027118
ACCTAAG12550.029.5907921
TTAGGTA6150.029.0325364
GTAATCA4400.028.8328489
GTGCAAG5250.028.652591
Produced by FastQC (version 0.11.2)