FastQCFastQC Report
Sun 14 Apr 2019
SRR6575177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827723
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50310.6078120337359237No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34040.41124869068516884No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30060.36316497185652685No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21520.2599903590935615No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15970.1929389421340231No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14240.1720382301808697No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12300.148600437586004No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12160.1469090504915292No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10670.1289078592717612No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10600.12806216572452378No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10480.12661240535783105No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9860.11912197679658533No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9770.1180346565215658No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9110.11006097450475581No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9010.10885284086584522No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8790.1061949468602419No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8530.10305379939907432No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC8420.10172485239627266No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8280.10003346530179782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.050.874351
GTATCAA19500.047.003471
ATCAACG20350.043.1767273
TCAACGC20350.043.1767274
CAACGCA21350.041.3744775
AACGCAG22100.039.9703646
TAGGTAT8500.038.1419335
AAGGGTA3950.038.064965
TTAGGTA8550.037.918884
GTGCAAG5500.037.6027831
CTTAGGT8500.037.036373
AGGGTAA4000.036.414496
TATCAAC24150.036.3960652
GTCTTAG9650.036.0441131
AGGTATA8800.035.239836
GGTAATC4300.033.8739438
GGTATAG9200.033.7076647
GTATAGT10350.033.5941668
GTACATG37800.033.2008671
TATAGTA10500.033.1142549
Produced by FastQC (version 0.11.2)