FastQCFastQC Report
Sun 14 Apr 2019
SRR6575165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348173
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25570.7344050227903944No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18320.5261752060039119No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14160.4066943731995301No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7930.22776033753335267No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6570.1886992960396125No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6010.17261533777748417No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT6010.17261533777748417No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5830.1674454940503715No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC5770.16572221280800062No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5410.15538252535377528No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA4770.13700085876848578No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4680.13441593690492945No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA4580.13154380150097797No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT4300.1235018223699138No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG4300.1235018223699138No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT4160.11948083280438172No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC4040.11603427031963995No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT3990.11459820261766421No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT3960.11373656199647876No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA3920.11258770783489817No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC3910.11230049429450302No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT3820.10971557243094668No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG3660.1051201557846243No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA3520.10109916621909222No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT3520.10109916621909222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950.060.4616781
GGTAATC1550.048.513618
GTATCAA7100.047.160111
TCAACGC7200.043.0872844
GTAATCA1750.042.9691969
AAGGGTA1750.042.9691965
ATCAACG7450.041.6414033
CAACGCA7700.040.8939825
AACGCAG7850.040.1125646
AGGGTAA1900.039.5768936
TTAGGTA5000.039.483624
TTAAGTA608.8066474E-539.170264
TATCAAC7950.039.022442
TAGGTAT5000.038.5385
GTCTTAG5400.038.4267581
AGGTATA5100.037.7823526
AGGGCTA757.4313266E-637.5980455
GGTATAG5400.037.423987
CTTAGGT5450.037.0859683
ACCAGAT9600.035.7377394
Produced by FastQC (version 0.11.2)