FastQCFastQC Report
Sun 14 Apr 2019
SRR6575153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698124
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10990.15742189066698753No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10130.14510316218895208No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9610.1376546286906051No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC7750.11101179733113316No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7520.10771725366840275No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7000.10026872017005574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.063.2477571
TCAACGC15050.043.7115024
ATCAACG15350.042.8572043
GGTATCA3100.042.448541
CAACGCA15600.042.170395
AACGCAG16350.040.523376
ACTACGC500.001703372737.5918923
TATCAAC17650.037.5467152
GTACATG27550.033.4349631
TACATGG28900.032.200672
ACATGGG28850.031.435163
ACGCAGA21450.030.8884437
CGCAGAG21450.030.8884438
ATAGACG1401.8926585E-830.207778
TAGACGA1354.6419518E-727.843859
GGTACCT2900.027.5457828
GCAGAGT24100.027.4900289
TAGGCGG1057.428364E-526.8513535
CATGGGG18850.026.1747264
ACTTAAG5050.026.057521
Produced by FastQC (version 0.11.2)