FastQCFastQC Report
Sun 14 Apr 2019
SRR6575152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA11700.15073434681783043No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10930.1408142231383664No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10800.13913939706261272No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC8350.10757536717340892No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8160.1051275444473074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14900.062.4753461
GGTATCA3750.050.1485561
TCAACGC19650.046.8795434
ATCAACG19850.046.4072043
CAACGCA20200.045.6031155
AACGCAG21050.043.761666
TATCAAC22150.041.1772882
AGGTATA2850.039.5782136
GGTATAG3150.037.30097
CGCAGAG26000.035.2493488
ACGCAGA26250.035.0926867
CTTAGGT3600.033.9438173
TAGGTAT3450.032.695055
GTATAGT6650.032.5106748
GTACATG32000.031.4407941
TTAGGTA3450.031.3327544
TACATGG32050.031.0983642
GCAGAGT29650.031.0685639
GTCTTAG4200.030.2234611
ACATGGG32950.030.096563
Produced by FastQC (version 0.11.2)