FastQCFastQC Report
Thu 30 Aug 2018
RXRa-TCP-rep-1_2_3_4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa-TCP-rep-1_2_3_4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67033978
Sequences flagged as poor quality0
Sequence length20-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT18558252.7684840663939116TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA11654801.7386406636944625TruSeq Adapter, Index 13 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT909220.13563569209632764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)